Introduction: Bioremediationmaybesustainablestrategyformitigatingheavy- metal contaminationinsoilsandrelatedenvironmentalmatrices. Methods: This paper,basedonPRISMA2020guidelinesanalyzes41 experimental studiespublishedbetween2022and2025,selectedfromScopus, WebofScience,andPubMed,withtheaimofcomparingbacterialtaxa, detoxificationmechanisms,andremediationperformance. Resultsanddiscussion: Bacillus and Pseudomonas werethemostfrequently investigatedgenera,with Bacillus spp. achievingthehighestremovalefficiencies (>80%–90%) throughbiosorptionandbiomineralizationmechanisms.On the otherhand, Pseudomonas spp. showedahighfunctionalandgenetic versatility,combiningeffluxsystems,enzymaticredoxtransformations,EPS production,andbiofilm-mediatedresilience,particularlyinmulti-metaland fluctuatingenvironmentalconditions.Medianremovalefficiencieswasat least 70%incontrolledlaboratoryassays,whileitwas10%–25%insoil. Despite methodologicalheterogeneity,theevidenceidentifies Pseudomonas as amechanisticbenchmarkgenusratherthanauniversallysuperior biosorbent, owingtoitsextensivegenomiccharacterizationandbroad detoxificationrepertoire.Thereviewhighlightstheneedforstandardized experimental protocols,integrationofmulti-omicsapproaches,andfield- scale validationtoimprovedatacomparabilityandtofacilitatethe translationofmicrobialbioremediationfromlaboratorystudiestosustainable environmentalapplications.
PRISMA-based review of Pseudomonas spp. in microbial heavy-metal bioremediation: mechanisms and taxonomy
De Santis, Alessandro;Corbo, Maria Rosaria;Racioppo, Angela;Bevilacqua, Antonio;Sinigaglia, Milena
2026-01-01
Abstract
Introduction: Bioremediationmaybesustainablestrategyformitigatingheavy- metal contaminationinsoilsandrelatedenvironmentalmatrices. Methods: This paper,basedonPRISMA2020guidelinesanalyzes41 experimental studiespublishedbetween2022and2025,selectedfromScopus, WebofScience,andPubMed,withtheaimofcomparingbacterialtaxa, detoxificationmechanisms,andremediationperformance. Resultsanddiscussion: Bacillus and Pseudomonas werethemostfrequently investigatedgenera,with Bacillus spp. achievingthehighestremovalefficiencies (>80%–90%) throughbiosorptionandbiomineralizationmechanisms.On the otherhand, Pseudomonas spp. showedahighfunctionalandgenetic versatility,combiningeffluxsystems,enzymaticredoxtransformations,EPS production,andbiofilm-mediatedresilience,particularlyinmulti-metaland fluctuatingenvironmentalconditions.Medianremovalefficiencieswasat least 70%incontrolledlaboratoryassays,whileitwas10%–25%insoil. Despite methodologicalheterogeneity,theevidenceidentifies Pseudomonas as amechanisticbenchmarkgenusratherthanauniversallysuperior biosorbent, owingtoitsextensivegenomiccharacterizationandbroad detoxificationrepertoire.Thereviewhighlightstheneedforstandardized experimental protocols,integrationofmulti-omicsapproaches,andfield- scale validationtoimprovedatacomparabilityandtofacilitatethe translationofmicrobialbioremediationfromlaboratorystudiestosustainable environmentalapplications.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
