Two commercial promising probiotic strains (Propionibacterium jensenii and Propionibacterium freudenreichii) were studied to evaluate two basic probiotic requisites (adhesion and survival during the transit into the gut); two commercial starter cultures (Lactobacillus plantarum and Lactobacillus delbrueckii subsp. bulgaricus) were used as references. As an additional goal, the technological robustness was evaluated as growth as a function of pH, temperature and salt. Adhesion was studied as biofilm formation and hydrophobicity, whereas a simplified protocol was used to simulate the transit into the gut, along with the assessment of survival at pH 2.5 and in the presence of 0.3% bile salts. Propionibacteria were hydrophobic and form biofilm on glass slides, thus confirming the correlation of these properties and with the ability to adhere. None of the strains survived after the simulation of the transit into the gut, but this property could be induced in propionibacteria using a simple protocol of adaptive evolution. This paper suggests the possibility of using hydrophobicity as a screening tool to assess adhesion in propionibacteria and highlights the necessity of using a complete protocol of simulation of the gut to study the resistance to the conditions of stomach and intestine to avoid false-positive results.
|Titolo:||How to routinely assess transition, adhesion and survival of probiotics into the gut: a case study on propionibacteria|
|Data di pubblicazione:||2018|
|Appare nelle tipologie:||1.1 Articolo in rivista|