Modelling the survival of starter lactic acid bacteria and Bifidobacterium bifidum in single and simultaneous cultures.

The inactivation kinetics at 4 1C of Bifidobacterium bifidum, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus, cultured alone or consociated in a laboratory medium (modified MRS broth), were modelled through the Weibull model and a second-order polynomial equation. The initial cell number of S. thermophilus and L. delbrueckii subsp. bulgaricus was approximately 6–7 log (CFU/ml); the viability loss after 30 days of storage was 2.87 and 1.99 log (CFU/l) for L. delbruckii subsp. bulgaricus and S. thermophilus, cultured alone, respectively; whereas the consociation of lactobacilli and streptococci with bifidobacteria reduced viability loss during storage (0.28 and 0.54 log CFU/l for lactobacilli and streptococci, respectively). Finally, the consociation of lactobacilli and streptococci with B. bifidum improved their oxygen uptake. & 2008 Published by Elsevier Ltd.