The aim of this study was to characterize the in vitro antimicrobial susceptibility profile of Coliform and Pseudomonas species strains to 10 antibiotics (Vancomycine, Ciprofloxine, Tetracyclin, Chloramphenicol , Erythromycin, Streptomycine, Gentamycine , Ampicilline, trimethoprimsulfamethoxazole and Clarithromycin) that could potentially be used in clinical therapy. MIC was determined for antibiotics in Mueller-Hinton (MH) agar plates, by using E-test strips (Liofilchem, Roseto degli Abruzzi, Italy). MIC was read after 24 h of incubation at 37°C. The both strains were more susceptible to Ciprofloxine (MIC values: Coliform, 0.002 μg/mL; and Pseudomonas, 0.047 μg/mL). No strain of Pseudomonas was found sensitive to Chloramphenicol, Erythromycin, Ampicilline, trimethoprim-sulfamethoxazole and Clarithromycin, contrary to coliform, which was found resistant to those antibiotics with MIC values ranged between 0.38 and 128 μg/mL . Gentamycine (MIC values: Coliform, 0.125μg/mL; and Pseudomonas, 0.50 μg/mL), Tetracyclin (MIC values: Coliform, 1.00 μg/mL; and Pseudomonas, 0.75 μg/mL) and Streptomycine (MIC values: Coliform, 0.75 μg/mL; and Pseudomonas, 2.5 μg/mL) were also active against Coliform and Pseudomonas strains, while resistance to Vancomycine (no inhibition) was observed in the two strains.
In vitro susceptibility of coliform and Pseudomonasspecie to some antibiotics by E-test strips
BEVILACQUA, ANTONIO;CORBO, MARIA ROSARIA;SINIGAGLIA, MILENA GRAZIA RITA;PIGNATIELLO, STEFANO
2014-01-01
Abstract
The aim of this study was to characterize the in vitro antimicrobial susceptibility profile of Coliform and Pseudomonas species strains to 10 antibiotics (Vancomycine, Ciprofloxine, Tetracyclin, Chloramphenicol , Erythromycin, Streptomycine, Gentamycine , Ampicilline, trimethoprimsulfamethoxazole and Clarithromycin) that could potentially be used in clinical therapy. MIC was determined for antibiotics in Mueller-Hinton (MH) agar plates, by using E-test strips (Liofilchem, Roseto degli Abruzzi, Italy). MIC was read after 24 h of incubation at 37°C. The both strains were more susceptible to Ciprofloxine (MIC values: Coliform, 0.002 μg/mL; and Pseudomonas, 0.047 μg/mL). No strain of Pseudomonas was found sensitive to Chloramphenicol, Erythromycin, Ampicilline, trimethoprim-sulfamethoxazole and Clarithromycin, contrary to coliform, which was found resistant to those antibiotics with MIC values ranged between 0.38 and 128 μg/mL . Gentamycine (MIC values: Coliform, 0.125μg/mL; and Pseudomonas, 0.50 μg/mL), Tetracyclin (MIC values: Coliform, 1.00 μg/mL; and Pseudomonas, 0.75 μg/mL) and Streptomycine (MIC values: Coliform, 0.75 μg/mL; and Pseudomonas, 2.5 μg/mL) were also active against Coliform and Pseudomonas strains, while resistance to Vancomycine (no inhibition) was observed in the two strains.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
