The invention relates to a diagnostic kit for multiple detection of 4 viruses of the Family Coronaviridae: HCoV, SARS-CoV, MERS-CoV and the SARS-CoV-2 viral strain that has caused a pandemic of the disease known as COVID-19. The kit uses a “One-Step” approach with quantitative gene amplification after backward transcription of the viral genome (rRT-PCR). In order to avoid potential false negatives, the invention contains a double control using Porcine Epidemic Diarrhoea Virus (PEDV-CoV) and Ribonuclease P (RNase P-RP).

Multiplex RT-PCR method and kit for the detection of multiple viruses of the Coronaviridae family: SARS-CoV2, SARS-CoV, HCoV, and MERS-CoV

Andrea Ballini;
2020-01-01

Abstract

The invention relates to a diagnostic kit for multiple detection of 4 viruses of the Family Coronaviridae: HCoV, SARS-CoV, MERS-CoV and the SARS-CoV-2 viral strain that has caused a pandemic of the disease known as COVID-19. The kit uses a “One-Step” approach with quantitative gene amplification after backward transcription of the viral genome (rRT-PCR). In order to avoid potential false negatives, the invention contains a double control using Porcine Epidemic Diarrhoea Virus (PEDV-CoV) and Ribonuclease P (RNase P-RP).
2020
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11369/466692
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact