The occurrence of biogenic amines (and especially histamine) in wine constitutes an increasing concern within the wine sector. The analysis of biogenic amines in food is usually carried out by HPLC and capillary electrophoresis. Detection relies upon a derivatization step, which is performed either before or after separation. HPLC remains the reference method for the determination of biogenic amines in wine according to the Organisation Internationale de la Vigne et du Vin. This technique, though precise and sensitive, is expensive and time-consuming, moreover HPLC analysis demands a costly apparatuses and trained personnel. The wine sector would benefit from the development of an alternative analytical technique for the determination of biogenic amines. This should be high-throughput, inexpensive and simple. Thin layer chromatography (TLC) could represent a good alternative to HPLC. TLC methods do not require heavy and/or costly equipment and allow for simultaneous analysis of several samples. The present work presents the development and validation of an analytical method that allows to determine the four main biogenic amines of wine (i.e. histamine, tyramine, putrescine and cadaverine) by means of TLC-densitometry.
High-throughput and inexpensive method for routine analysis of biogenic amines in wines
BENEDUCE, LUCIANO;SPANO, GIUSEPPE;
2012-01-01
Abstract
The occurrence of biogenic amines (and especially histamine) in wine constitutes an increasing concern within the wine sector. The analysis of biogenic amines in food is usually carried out by HPLC and capillary electrophoresis. Detection relies upon a derivatization step, which is performed either before or after separation. HPLC remains the reference method for the determination of biogenic amines in wine according to the Organisation Internationale de la Vigne et du Vin. This technique, though precise and sensitive, is expensive and time-consuming, moreover HPLC analysis demands a costly apparatuses and trained personnel. The wine sector would benefit from the development of an alternative analytical technique for the determination of biogenic amines. This should be high-throughput, inexpensive and simple. Thin layer chromatography (TLC) could represent a good alternative to HPLC. TLC methods do not require heavy and/or costly equipment and allow for simultaneous analysis of several samples. The present work presents the development and validation of an analytical method that allows to determine the four main biogenic amines of wine (i.e. histamine, tyramine, putrescine and cadaverine) by means of TLC-densitometry.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
