Polyphenols are a heterogeneous group of widespread bioactive plant secondary metabolites, comprising simple molecules as well as condensed or polymeric forms that are grouped into several classes according to their chemical structure. The large number of these compounds possessing very different physico-chemical characteristics represents a challenge in the development of efficient multiclass extraction, separation and detection methods aimed at the characterization of polyphenolic profiles in different real matrices, in particular those belonging to vegetable by-products that can be used for the development of functional food. In this work a multiclass analysis approach based on a simple ultrasound assisted solid-liquid extraction followed by a HPLC separation with a multi-wavelength UV detection is presented. In the development of the extraction method the effect of important parameters (i.e. time, temperature, acidification, solvent, filter) on both the stability of analytes and recovery have been evaluated. The use of a C18 core–shell column and the development of an efficient multi-step gradient (combination of concave and linear ramps) allowed the separation in less than 21 min of 38 polyphenols belonging to the classes of phenolic acids, flavonoids and other phenols. The determination of polyphenols has been achieved at low ppm levels with recoveries above 80 %. The potential of the proposed method has been also assessed by the analysis of dried vegetable by-products.
Development of an analytical method based on ultrasound assisted extraction and HPLC separation with multi-wavelength UV detection of multiclass polyphenols in vegetable by-products
C. De Nido
;C. Palermo;D. Centonze
2023-01-01
Abstract
Polyphenols are a heterogeneous group of widespread bioactive plant secondary metabolites, comprising simple molecules as well as condensed or polymeric forms that are grouped into several classes according to their chemical structure. The large number of these compounds possessing very different physico-chemical characteristics represents a challenge in the development of efficient multiclass extraction, separation and detection methods aimed at the characterization of polyphenolic profiles in different real matrices, in particular those belonging to vegetable by-products that can be used for the development of functional food. In this work a multiclass analysis approach based on a simple ultrasound assisted solid-liquid extraction followed by a HPLC separation with a multi-wavelength UV detection is presented. In the development of the extraction method the effect of important parameters (i.e. time, temperature, acidification, solvent, filter) on both the stability of analytes and recovery have been evaluated. The use of a C18 core–shell column and the development of an efficient multi-step gradient (combination of concave and linear ramps) allowed the separation in less than 21 min of 38 polyphenols belonging to the classes of phenolic acids, flavonoids and other phenols. The determination of polyphenols has been achieved at low ppm levels with recoveries above 80 %. The potential of the proposed method has been also assessed by the analysis of dried vegetable by-products.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
