BACKGROUND Stegobium paniceum (Coleoptera, Anobiidae) is an important pest of stored products causing severe damage to dried Chinese medicinal plant materials (CMPMs). Plant volatiles play an important role in host-searching of insects. The olfactory responses of S. paniceum to the most abundant volatile components of some drugstore attractant CMPMs such as Panax notoginseng, Angelica sinensis, Gastrodia elata and Peucedanum praeruptorum, namely falcarinol, 3-n-butylphthalide, p-cresol and beta-pinene, respectively, were studied by electroantennography (EAG) and behavioural bioassays in six- and four-arm olfactometers. RESULTS EAG recordings showed that male and female antennae are able to perceive the test compounds in a wide range of concentrations and in a dose-dependent manner. Moreover, for each dose of different compounds tested, no significant differences were found between the mean male and female EAG responses. In six-arm olfactometer bioassays, S. paniceum exhibited positive responses to falcarinol, 3-n-butylphthalide, p-cresol and beta-pinene at doses of 1, 10, 100, 500 and 1000 mu g. The most attractive dose was 500 mu g for falcarinol, 100 mu g for 3-n-butylphthalide, 500 mu g for p-cresol and 1000 mu g for beta-pinene. Olfactory preferences of S. paniceum, based on comparison of these four compounds at their optimally attractive concentrations in a four-arm olfactometer, were 3-n-butylphthalide > p-cresol > falcarinol > beta-pinene. CONCLUSION The results indicated that the four volatiles of CMPMs are perceived by the peripheral olfactory system of S. paniceum adults and are able to individually elicit a positive chemotaxis in S. paniceum adults confirming the role of chemical cues in host-plant detection and selection of this pest. Further field studies are needed to evaluate the potential of the attractive compounds identified in this study, particularly 3-n-butylphthalide, to be applied as a novel monitoring and control tool against this storage-beetle pest. (c) 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Electrophysiological and behavioural responses of Stegobium paniceum to volatile compounds from Chinese medicinal plant materials
Pistillo, Onofrio Marco;D'Isita, Ilaria;Germinara, Giacinto Salvatore
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2022-01-01
Abstract
BACKGROUND Stegobium paniceum (Coleoptera, Anobiidae) is an important pest of stored products causing severe damage to dried Chinese medicinal plant materials (CMPMs). Plant volatiles play an important role in host-searching of insects. The olfactory responses of S. paniceum to the most abundant volatile components of some drugstore attractant CMPMs such as Panax notoginseng, Angelica sinensis, Gastrodia elata and Peucedanum praeruptorum, namely falcarinol, 3-n-butylphthalide, p-cresol and beta-pinene, respectively, were studied by electroantennography (EAG) and behavioural bioassays in six- and four-arm olfactometers. RESULTS EAG recordings showed that male and female antennae are able to perceive the test compounds in a wide range of concentrations and in a dose-dependent manner. Moreover, for each dose of different compounds tested, no significant differences were found between the mean male and female EAG responses. In six-arm olfactometer bioassays, S. paniceum exhibited positive responses to falcarinol, 3-n-butylphthalide, p-cresol and beta-pinene at doses of 1, 10, 100, 500 and 1000 mu g. The most attractive dose was 500 mu g for falcarinol, 100 mu g for 3-n-butylphthalide, 500 mu g for p-cresol and 1000 mu g for beta-pinene. Olfactory preferences of S. paniceum, based on comparison of these four compounds at their optimally attractive concentrations in a four-arm olfactometer, were 3-n-butylphthalide > p-cresol > falcarinol > beta-pinene. CONCLUSION The results indicated that the four volatiles of CMPMs are perceived by the peripheral olfactory system of S. paniceum adults and are able to individually elicit a positive chemotaxis in S. paniceum adults confirming the role of chemical cues in host-plant detection and selection of this pest. Further field studies are needed to evaluate the potential of the attractive compounds identified in this study, particularly 3-n-butylphthalide, to be applied as a novel monitoring and control tool against this storage-beetle pest. (c) 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
