MicroRNAs (miRNAs) regulate diverse biological processes by inhibiting translation or inducing degradation of target mRNAs. miR-145 is a candidate tumor suppressor in colorectal carcinoma (CRC). Colorectal carcinogenesis involves deregulation of cellular processes controlled by a number of intertwined chief transcription factors, such as PPAR gamma and SOX9. Since PPAR family members are able to modulate complex miRNAs networks, we hypothesized a role of miRNA-145 in the interaction between PPAR gamma and SOX9 in colorectal carcinogenesis. To address this issue, we evaluated gene expression in tissue specimens of CRC patients and we took advantage of in vitro models represented by CRC derived cell lines (CaCo2, SW480, HCT116, and HT-29), employing PPAR gamma activation and/or miRNA-145 ectopic overexpression to analyze how their interplay impact the expression of SOX9 and the development of a malignant phenotype. Results: PPAR gamma regulates the expression of miR-145 by directly binding to a PPAR response element (PPRE) in its promoter at -1207/- 1194 bp from the transcription start site. The binding is essential for miR-145 upregulation by PPAR gamma upon rosiglitazone treatment. Ectopic expression of miR-145, in turn, regulates SOX9 expression through the binding to specific seed motifs. The PPAR gamma-miR-145-S0X9 axis overarches cell cycle progression, invasiveness and differentiation of CRC derived cell lines. Together, these results suggest that miR-145 is a novel target of PPAR gamma, acts as a tumor suppressor in CRC cell lines and is a key regulator of intestinal cell differentiation by directly targeting SOX9, a marker of undifferentiated progenitors in the colonic crypts.

Peroxisome proliferator-activated receptor γ-mediated induction of microRNA-145 opposes tumor phenotype in colorectal cancer

Panza, A.;Valvano, M. R.;Colangelo, T.;Merla, G.;Prattichizzo, C.;
2014-01-01

Abstract

MicroRNAs (miRNAs) regulate diverse biological processes by inhibiting translation or inducing degradation of target mRNAs. miR-145 is a candidate tumor suppressor in colorectal carcinoma (CRC). Colorectal carcinogenesis involves deregulation of cellular processes controlled by a number of intertwined chief transcription factors, such as PPAR gamma and SOX9. Since PPAR family members are able to modulate complex miRNAs networks, we hypothesized a role of miRNA-145 in the interaction between PPAR gamma and SOX9 in colorectal carcinogenesis. To address this issue, we evaluated gene expression in tissue specimens of CRC patients and we took advantage of in vitro models represented by CRC derived cell lines (CaCo2, SW480, HCT116, and HT-29), employing PPAR gamma activation and/or miRNA-145 ectopic overexpression to analyze how their interplay impact the expression of SOX9 and the development of a malignant phenotype. Results: PPAR gamma regulates the expression of miR-145 by directly binding to a PPAR response element (PPRE) in its promoter at -1207/- 1194 bp from the transcription start site. The binding is essential for miR-145 upregulation by PPAR gamma upon rosiglitazone treatment. Ectopic expression of miR-145, in turn, regulates SOX9 expression through the binding to specific seed motifs. The PPAR gamma-miR-145-S0X9 axis overarches cell cycle progression, invasiveness and differentiation of CRC derived cell lines. Together, these results suggest that miR-145 is a novel target of PPAR gamma, acts as a tumor suppressor in CRC cell lines and is a key regulator of intestinal cell differentiation by directly targeting SOX9, a marker of undifferentiated progenitors in the colonic crypts.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11369/440409
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