In this paper allosteric interactions in protonmotive heme aa3terminal oxidases of the respiratory chain are dealt with. The different lines of evidence supporting the key role of H+/e-coupling (redox Bohr effect) at the low spin heme a in the proton pump of the bovine oxidase are summarized. Results are presented showing that the I-R54M mutation in P. denitrificans aa3oxidase, which decreases by more than 200 mV the Emof heme a, inhibits proton pumping. Mutational aminoacid replacement in proton channels, at the negative (N) side of membrane-inserted prokaryotic aa3oxidases, as well as Zn2 +binding at this site in the bovine oxidase, uncouples proton pumping. This effect appears to result from alteration of the structural/functional device, closer to the positive, opposite (P) surface, which separates pumped protons from those consumed in the reduction of O2to 2 H2O. This article is part of a Special Issue entitled: Respiratory Oxidases. © 2011 Elsevier B.V. All rights reserved.
Allosteric interactions and proton conducting pathways in proton pumping aa3oxidases: Heme a as a key coupling element
Capitanio, Nazzareno;
2012-01-01
Abstract
In this paper allosteric interactions in protonmotive heme aa3terminal oxidases of the respiratory chain are dealt with. The different lines of evidence supporting the key role of H+/e-coupling (redox Bohr effect) at the low spin heme a in the proton pump of the bovine oxidase are summarized. Results are presented showing that the I-R54M mutation in P. denitrificans aa3oxidase, which decreases by more than 200 mV the Emof heme a, inhibits proton pumping. Mutational aminoacid replacement in proton channels, at the negative (N) side of membrane-inserted prokaryotic aa3oxidases, as well as Zn2 +binding at this site in the bovine oxidase, uncouples proton pumping. This effect appears to result from alteration of the structural/functional device, closer to the positive, opposite (P) surface, which separates pumped protons from those consumed in the reduction of O2to 2 H2O. This article is part of a Special Issue entitled: Respiratory Oxidases. © 2011 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.