L-Glutamate (Glu) is a well-known flavour enhancer that is present in several foodstuffs either as an additive or a natural compound. Glu monitoring is an important issue since the excessive intake of this flavour enhancer can cause allergic and neurotoxic effects. Glu is currently determined by chromatographic [1] or capillary electrophoretic [2] methods, which require extensive sample pre-treatment and expensive equipments. A suitable alternative is represented by amperometric biosensors, low cost devices that could provide specific, rapid and repetitive analyses of complex matrices. In the last decade a number of biosensors for glutamate detection have been proposed [3-7], but the above mentioned requirements have not been completely met. In order to face these problems a proper selection of the electrode material and the use of permselective films are required [8].This work describes the development and optimization of an amperometric biosensor for glutamate monitoring in foodstuffs. The biosensor is based on glutamate oxidase (GLOD) immobilized by a gel of bovine serum albumine and glutharaldeyde onto a platinum electrode modified with a permselective overoxidized polypyrrole film. Different experimental conditions have been tested for the enzyme immobilization, and the optimized biosensor, integrated in a flow injection system, has been characterized in terms of linearity, LOD, LOQ, repeatability and stability of response. The excellent anti-interference characteristics towards the main interferents present in real food matrices have allowed the application of the biosensor in the accurate monitoring of Glu in different kind of foodstuffs. [1] T.P. Piepponen, A. Skujins, J. Chromatogr. 757(2001)277; [2] M.W. Lada, R.T. Kennedy, Anal. Chem. 68(1996)2790; [3] N.H. Kwon, M.S. Won, E.S. Choe, Y.B. Shim, Anal. Chem. 77(2005)4854; [4] F. Tian, A.V. Gourine, R.T.R. Huckstepp, N. Dale, Anal. Chim. Acta 645(2009)86; [5] M. Ammam, J. Fransaer, Biosens. Bioelectron. 25(2010)1597; [6] O. Frey, Holtzman T, McNamara RM, Theobald DEH, van der Wal PD, de Rooij NF, Dalley JW, Koudelka-Hep M., Biosens. Bioelectron. 26(2010)477; [7] Jamal M, Xu J, Razeeb KM, Biosens. Bioelectron. 26(2010)1420; [8] D. Carelli, D. Centonze, C. Palermo, M. Quinto, T. Rotunno, Biosens. Bioelectron. 23(2007)640.
SENSITIVE AND INTERFERENCE-FREE GLUTAMATE AMPEROMETRIC BIOSENSOR FOR THE MONITORING OF FOODSTUFFS
CENTONZE, DIEGO;NARDIELLO, DONATELLA;PALERMO, CARMEN;QUINTO, MAURIZIO
2013-01-01
Abstract
L-Glutamate (Glu) is a well-known flavour enhancer that is present in several foodstuffs either as an additive or a natural compound. Glu monitoring is an important issue since the excessive intake of this flavour enhancer can cause allergic and neurotoxic effects. Glu is currently determined by chromatographic [1] or capillary electrophoretic [2] methods, which require extensive sample pre-treatment and expensive equipments. A suitable alternative is represented by amperometric biosensors, low cost devices that could provide specific, rapid and repetitive analyses of complex matrices. In the last decade a number of biosensors for glutamate detection have been proposed [3-7], but the above mentioned requirements have not been completely met. In order to face these problems a proper selection of the electrode material and the use of permselective films are required [8].This work describes the development and optimization of an amperometric biosensor for glutamate monitoring in foodstuffs. The biosensor is based on glutamate oxidase (GLOD) immobilized by a gel of bovine serum albumine and glutharaldeyde onto a platinum electrode modified with a permselective overoxidized polypyrrole film. Different experimental conditions have been tested for the enzyme immobilization, and the optimized biosensor, integrated in a flow injection system, has been characterized in terms of linearity, LOD, LOQ, repeatability and stability of response. The excellent anti-interference characteristics towards the main interferents present in real food matrices have allowed the application of the biosensor in the accurate monitoring of Glu in different kind of foodstuffs. [1] T.P. Piepponen, A. Skujins, J. Chromatogr. 757(2001)277; [2] M.W. Lada, R.T. Kennedy, Anal. Chem. 68(1996)2790; [3] N.H. Kwon, M.S. Won, E.S. Choe, Y.B. Shim, Anal. Chem. 77(2005)4854; [4] F. Tian, A.V. Gourine, R.T.R. Huckstepp, N. Dale, Anal. Chim. Acta 645(2009)86; [5] M. Ammam, J. Fransaer, Biosens. Bioelectron. 25(2010)1597; [6] O. Frey, Holtzman T, McNamara RM, Theobald DEH, van der Wal PD, de Rooij NF, Dalley JW, Koudelka-Hep M., Biosens. Bioelectron. 26(2010)477; [7] Jamal M, Xu J, Razeeb KM, Biosens. Bioelectron. 26(2010)1420; [8] D. Carelli, D. Centonze, C. Palermo, M. Quinto, T. Rotunno, Biosens. Bioelectron. 23(2007)640.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
