The production of micropropagated transplants in early artichoke cuItivars is neglected by the loss of earliness and by the very low multiplication rate. The high concentration of hormones and nutrients and the high number of subcuItures are in contrast with the necessity to avoid genetic variations to which the loss of the earliness trait is attributed. In addition the contamination of the explants and the occurrence of oxidizating problems during initiation are frequentely repoted. Two pre-treatment protocol (Pl and P2) and four culture media were tested in initiation (El, E2, E3 and E4) and in multiplication (MI, M2, M3 and M4) phase in order to improve growth and multiplication rate of 'Violet du Provence' explants. Apices were treated with sodium hypocloride, before (Pl) and after (P2) the antioxidant solution. During initiation phase colture media were characterized by high (E2 and E3) or low (El and E4) hormonal level, while during muItiplication phase M2 was characterized by a higher content of hormones compared to MI, M3 and M4. Pl protocol determined a sensible reduction of the explant oxidation (11.6 vs 37.7%). The substrates E3 and E4 registered the highest growth values up to the last week; due to the rapid height increase observed in the last part of the initiation, the explants raised on El were 18% higher of that observed in E3 and E4 and 31% higher of those produced in E2. The M3 substrate determined the higher growth increase (208.9 mm), especially if compared to M4 (+57.2%). The number of shoots formed from every initial bud (multiplication index, 1M) was 4.5 in M3 with an average height of about 42 mm.

Micropropagation of the early artichoke cultivar 'Violet du Provence'

ELIA, ANTONIO;CONVERSA, GIULIA;LOTTI, CONCETTA
2007-01-01

Abstract

The production of micropropagated transplants in early artichoke cuItivars is neglected by the loss of earliness and by the very low multiplication rate. The high concentration of hormones and nutrients and the high number of subcuItures are in contrast with the necessity to avoid genetic variations to which the loss of the earliness trait is attributed. In addition the contamination of the explants and the occurrence of oxidizating problems during initiation are frequentely repoted. Two pre-treatment protocol (Pl and P2) and four culture media were tested in initiation (El, E2, E3 and E4) and in multiplication (MI, M2, M3 and M4) phase in order to improve growth and multiplication rate of 'Violet du Provence' explants. Apices were treated with sodium hypocloride, before (Pl) and after (P2) the antioxidant solution. During initiation phase colture media were characterized by high (E2 and E3) or low (El and E4) hormonal level, while during muItiplication phase M2 was characterized by a higher content of hormones compared to MI, M3 and M4. Pl protocol determined a sensible reduction of the explant oxidation (11.6 vs 37.7%). The substrates E3 and E4 registered the highest growth values up to the last week; due to the rapid height increase observed in the last part of the initiation, the explants raised on El were 18% higher of that observed in E3 and E4 and 31% higher of those produced in E2. The M3 substrate determined the higher growth increase (208.9 mm), especially if compared to M4 (+57.2%). The number of shoots formed from every initial bud (multiplication index, 1M) was 4.5 in M3 with an average height of about 42 mm.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11369/16154
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